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        Your Position: Home > News

        The key to potato tissue culture is sterilization

        2018/7/28      view:

        Technical literature on potato tissue culture:

            Fungus contamination is common in potato tissue culture, so it is a key measure to improve the yield and quality of micropotato.

            1. Causes of fungus contamination of potato tissue culture seedlings
            (1)Medium pollution: it may be caused by incomplete sterilization, aging sealing material and poor sealing performance;
            (2)Tissue culture seedlings with bacteria: the main reason is that the inoculation materials themselves contain bacteria, but the amount of bacteria in the early stage is not obvious, and the amount of bacteria after inoculation is rich and polluted;
            (3)Excessive concentration of fungal spores in the inoculation room or culture room;
            (4)Incomplete sterilization of inoculation equipment;
            (5)The ultra-clean platform is unclean.

            2. Measures to prevent and control fungus contamination of potato tissue culture seedlings
            (1)Regularly check the sterilization quality of the sterilization pot, and if there is any problem, repair it immediately;The sterilizing materials in the sterilizing pot should not be piled up to the full, so as not to affect the steam circulation and heat exchange;The cold air should be thoroughly discharged so as not to cause the actual temperature in the sterilizer pot to be inconsistent with the temperature on the pressure gauge.You can't go up and down too fast.
            (2)For pollution caused by excessive concentration of fungal spores in the inoculation room or culture room, 75% ethanol solution should be used to spray regularly to reduce dust and kill spores in the air to keep the room clean.
            (3) The inoculation equipment shall be put into the sterilization pot regularly for sterilization, and tools shall be changed frequently during inoculation to avoid cross-contamination, and the inoculation equipment shall be thoroughly sterilized frequently during inoculation.
            (4)Clean and repair the ultra-clean platform regularly.Before sterilization, the ultraviolet lamp in the ultra-clean table should be on for more than 30 minutes. The blank medium on the ultra-clean table should not be piled up too much during inoculation, otherwise the airflow will not be free.
            (5)The inoculation process should be carried out in strict accordance with the operating rules, and the hands should be wiped with 75% ethanol frequently.

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